Figure 1. CCP in pachytene bivalents of B. sylvaticum (2n = 18) using BAC pools spanning the short (green fluorescence) and long (red fluorescence) arms of all five (Bd1-Bd5) B. distachyon chromosomes.

Bd1- (A), Bd2- (B), Bd3- (C), Bd4- (D), Bd5-specific probes (E). Painted bivalents or their fragments in the photomicrographs are numbered arbitrarily using Roman numerals, which correspond to those on the idiograms. Bivalents that cannot be traced end- to-end are marked by dotted open-ended lines. White arrows indicate yellow fluorescence caused by the hybridisation of non-specific repeats common to the two chromosome arms. Chromosomes in Figures 1–7 are counterstained with DAPI. Relative lengths of chromosomes in the idiograms are only approximate. Bar: 10 µm.