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50 ng purified GST-p62 was exposed to different molar ratios of (A) NaOCl or (B) H2O2 for 1 h at 37°C, or to different concentrations of (C) peroxynitrite for 5 min at room temperature or (D) DEA/NONOate for 20 min at room temperature. All reactions were immunoblotted for p62. All images presented are representative of at least 3 independent experiments.
