Figure 4.

Effect of As₄S₄ on the release of LDH and the activation of caspase-3 and −9. (A) Subsequent to exposure of A375 and MKN45 cells to the respective IC₅₀s of As₄S₄ for 36 h, and exposure of L02 cells to 10 μg/ml As₄S₄, the leakage of LDH from cells was analyzed. (B) Caspase-3 and −9 activation was analyzed in MKN45 cells treated with different concentrations of As₄S₄ for 36 h: Control, 0 μg/ml; L, 0.25 × IC₅₀; M, 0.5 × IC₅₀ and H, 1 × IC₅₀. Data are expressed as the mean ± standard deviation; ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. control. As₄S₄, arsenic sulfide; LDH, lactate dehydrogenase; L, low; M, medium; H, high.