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A cell lysate fractionation of normal cells, Flag-TDP-12xQ/N HEK293 and Flag-TDP-43 F4L stable cell lines induced or not with tetracycline. The soluble (S) and insoluble (P) cell fractions were separated by ultracentrifugation, and the corresponding Flag-tagged proteins and endogenous TDP-43 were detected by western blot using an anti-TDP-43 antibody.
