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An immunofluorescence of HEK293 cells expressing the Flag-TDP-12xQ/N mutants proteins described in Figure 4A. The assay was performed using anti-Flag (red) and anti-TDP-43 (green) antibodies. The cell nuclei were stained with the reagent TOPRO-3. A merge between anti-Flag/anti-TDP-43 antibodies or anti-Flag/anti-TDP-43/TOPRO-3 is also shown.
