Table 1.
Genotyping of several mutant alleles common in inbred mouse strains did not explain the modifying effect observed in the different Crb1rd8/rd8 homozygous lines
| Candidate alleles | Crb1rd8/rd8/J | C57BL/6 Crb1rd8/rd8(1) | C57BL/6 Crb1rd8/rd8(2) | C57BL/6J | Balb/c | 129 S2/Sv |
|---|---|---|---|---|---|---|
| Crb1rd8 (c.3481delC)* | 100% (10/10) | 100% (7/7) | 100% (15/15) | 0% (0/4) | 0% (0/4) | 0% (0/4) |
| Pde6brd1 (c.1041C>A) | 0% (0/10) | 0% (0/7) | 0% (0/15) | 0% (0/4) | 0% (0/4) | 0% (0/4) |
| Gnat2 (c.518A>G) | 0% (0/8) | 0% (0/5) | 0% (0/15) | 0% (0/3) | 0% (0/3) | 0% (0/3) |
| Gnat2Cpfl3 (c.598G>A) | 0% (0/10) | 0% (0/7) | 0% (0/15) | 0% (0/4) | 0% (0/4) | 0% (0/4) |
| RPE65Leu450/Leu450 or RPE65Met450/Met450 (c.1348C>A) |
100% (10/10) RPE65Met450 |
100% (7/7) RPE65Met450 |
100% (15/15) RPE65Met450 |
100% (4/4) RPE65Met450 |
100% (4/4) RPE65Leu450 |
100% (4/4) RPE65Leu450 |
Percentage and absolute number of the genotyping result for the respective mutant and modifying allele. These results were obtained by DNA sequencing of individual animals from the three homozygous Crb1rd8/rd8/J, C57BL/6 Crb1rd8/rd8(1) and C57BL/6 Crb1rd8/rd8(2) mouse lines (n = 10 each) and from wild-type animals from inbred lines with different genetic backgrounds (C57BL/6J, Balb/c and 129 S2/Sv) that served as controls (n = 4 each). In addition to the represented animals in this table all animals used in this study were genotyped for the Crb1rd8 allele.