Table 5.

Real-time PCR assays with corresponding primers and Roche FAM-labelled universal probes

Assay	Primer sequences	Roche universal probe library
Ccl2	Forw: catccacgtgttggctca Rev: gatcatcttgctggtgaatgagt	#62
Ccr2	Forw: acctgtaaatgccatgcaagt Rev: tgtcttccatttcctttgatttg	#27
Crb1	Forw: caactcagcccatgtcctc Rev: aaaacagcctttgcgataca	#32
Crb2	Forw: caggattctctggccagttc Rev: caggcactgctacctccag	#34
Cx3cl1	Forw: ccgaattcctgcactcca Rev: catgatttcgcatttcgtca	#66
Cx3cr1	Forw: ggagtctgcgtgagactgg Rev: agcagatgggaagggaactt	#4
Nos2	Forw: ctttgccacggacgagac Rev: tcattgtactctgagggctgac	#13
Arg1	Forw: gaatctgcatgggcaacc Rev: gaatcctggtacatctgggaac	#2
Tgfb1	Forw: tcagacattcgggaagcagt Rev: acgccaggaattgttgctat	#56
Actb	Forw: aaggccaaccgtgaaaagat Rev: gtcgtacgaccagaggcatac	#56

Assays were designed using the online Assay Design Centre (www.roche-applied-science.com).