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. 2014 Oct 29;11(2):865–870. doi: 10.3892/mmr.2014.2807

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Effect of high glucose on osteoclast formation in RAW264.7 cells. The Raw264.7 cells were seeded at a density of 2×10⁴ cells/well in 24-well plates and cultured with RANKL and different doses (5.6 and 20.2 mM) of D-glucose or mannitol for 4 days. (A) High glucose culture with 20.2 mM D-glucose significantly decreased the number of TRAP-positive multinucleated cells compared with the control culture in 5.6 mM D-glucose (^**P<0.01; n=4). No significant differences were observed in the osmotic control culture compared with the control culture. Data are expressed as the mean ± standard deviation. (B) Images of the TRAP-positive multinucleated cells were captured under a microscope (magnification, ×200). RANKL, receptor activator of nuclear factor-κB ligand; TRAP, tartrate-resistant acid phosphatase.

Effect of high glucose on osteoclast formation in RAW264.7 cells. The Raw264.7 cells were seeded at a density of 2×10⁴ cells/well in 24-well plates and cultured with RANKL and different doses (5.6 and 20.2 mM) of D-glucose or mannitol for 4 days. (A) High glucose culture with 20.2 mM D-glucose significantly decreased the number of TRAP-positive multinucleated cells compared with the control culture in 5.6 mM D-glucose (^**P<0.01; n=4). No significant differences were observed in the osmotic control culture compared with the control culture. Data are expressed as the mean ± standard deviation. (B) Images of the TRAP-positive multinucleated cells were captured under a microscope (magnification, ×200). RANKL, receptor activator of nuclear factor-κB ligand; TRAP, tartrate-resistant acid phosphatase.