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. 2014 Oct 29;11(2):865–870. doi: 10.3892/mmr.2014.2807

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Effect of high glucose on the mRNA expression levels of c-fos and NFATc1. RAW264.7 cells were seeded at a density of 1×10⁵ cells/well in 6-well plates and cultured with RANKL and different doses (5.6 and 20.2 mM) of D-glucose or mannitol for 5 days. The mRNA expression levels of (A) c-fos and (B) NFATc1 were analyzed using reverse transcription-quantitative polymerase chain reaction. Data are expressed as the mean ± standard deviation of the quantification of target expression relative to the untreated group following normalization against β-actin (2^−ΔΔCt method). The untreated group were defined as the standard (=1). ^*P<0.05 and ^**P<0.01 vs 5.6 mM glucose-treated cells. RANKL, receptor activator of nuclear factor-κB ligand; NFATc1, nuclear factor of activated T cells c1.

Effect of high glucose on the mRNA expression levels of c-fos and NFATc1. RAW264.7 cells were seeded at a density of 1×10⁵ cells/well in 6-well plates and cultured with RANKL and different doses (5.6 and 20.2 mM) of D-glucose or mannitol for 5 days. The mRNA expression levels of (A) c-fos and (B) NFATc1 were analyzed using reverse transcription-quantitative polymerase chain reaction. Data are expressed as the mean ± standard deviation of the quantification of target expression relative to the untreated group following normalization against β-actin (2^−ΔΔCt method). The untreated group were defined as the standard (=1). ^*P<0.05 and ^**P<0.01 vs 5.6 mM glucose-treated cells. RANKL, receptor activator of nuclear factor-κB ligand; NFATc1, nuclear factor of activated T cells c1.