Table 1.

Thermodynamic parameters for the binding of wildtype WW1 domain of YAP2 to PPXY-containing peptides derived from various cognate ligands

Ligand	Sequence	Kd / µM	ΔH / kcal.mol−1	TΔS / kcal.mol−1	ΔG / kcal.mol−1	ΔCp / cal.mol−1.K−1
ErbB4		36 ± 3	−14.20 ± 0.28	−8.13 ± 0.24	−6.07 ± 0.05	−505 ± 42
LATS1		18 ± 2	−11.50 ± 0.57	−5.00 ± 0.64	−6.50 ± 0.07	−486 ± 33
p73		50 ± 5	−7.02 ± 0.18	−1.13 ± 0.24	−5.88 ± 0.06	−143 ± 30
PTCH1		67 ± 3	−9.62 ± 0.82	−4.24 ± 1.31	−5.67 ± 0.06	−373 ± 81
PTPN14		62 ± 8	−12.85 ± 0.35	−7.15 ± 0.36	−5.75 ± 0.08	−360 ± 14
RUNX1		59 ± 6	−11.40 ± 0.42	−5.62 ± 0.48	−5.78 ± 0.06	−415 ± 78
SMAD7		51 ± 6	−14.95 ± 0.49	−9.08 ± 0.57	−5.87 ± 0.07	−270 ± 57
TMG2		66 ± 9	−13.30 ± 0.42	−7.58 ± 0.51	−5.72 ± 0.08	−375 ± 92
WBP1		130 ± 6	−14.15 ± 0.35	−8.84 ± 0.38	−5.31 ± 0.03	−370 ± 14
WBP2		61 ± 6	−9.52 ± 0.32	−3.75 ± 0.26	−5.76 ± 0.06	−255 ± 64

The consensus residues within the PPXY motif of each peptide are colored red for clarity. All parameters were obtained from ITC measurements at 25°C and pH 7. All binding stoichiometries were fixed to unity. Errors were calculated from at least three independent measurements to one standard deviation.