. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: FEBS J. 2014 Nov 4;281(24):5532–5551. doi: 10.1111/febs.13095

Table 5.

Thermodynamic parameters for the binding of wildtype WW1-WW2 tandem module of YAP2 to PPXY-containing peptides derived from various cognate ligands

Ligand	K_d / µM	ΔH / kcal.mol⁻¹	TΔS / kcal.mol⁻¹	ΔG / kcal.mol⁻¹	ΔC_p / cal.mol⁻¹.K⁻¹
ErbB4	72 ± 13	−13.55 ± 0.92	−7.89 ± 1.02	−5.66 ± 0.11	−52 ± 8
LATS1	32 ± 6	−9.67 ± 0.28	−3.51 ± 0.41	−6.15 ± 0.12	−5 ± 3
p73	108 ± 10	−10.70 ± 0.57	−5.28 ± 0.62	−5.42 ± 0.05	−70 ± 42
PTCH1	133 ± 12	−13.95 ± 0.35	−8.65 ± 0.30	−5.30 ± 0.05	−55 ± 7
PTPN14	112 ± 8	−19.70 ± 0.99	−14.30 ± 1.03	−5.40 ± 0.04	−48 ± 11
RUNX1	98 ± 12	−18.00 ± 0.57	−12.52 ± 0.64	−5.48 ± 0.07	−53 ± 25
SMAD7	129 ± 8	−16.40 ± 0.57	−11.09 ± 0.60	−5.31 ± 0.04	−38 ± 32
TMG2	120 ± 16	−17.75 ± 0.49	−12.39 ± 0.41	−5.36 ± 0.08	−45 ± 21
WBP1	232 ± 33	−17.55 ± 1.48	−12.58 ± 1.57	−4.97 ± 0.09	−100 ± 28
WBP2	132 ± 13	−13.35 ± 0.64	−8.05 ± 0.70	−5.30 ± 0.06	−90 ± 71

Note that in the wildtype WW1-WW2 tandem module, both WW domains are capable of ligand binding. The consensus residues within the PPXY motif of each peptide are colored red for clarity. All parameters were obtained from ITC measurements at 25°C and pH 7. All binding stoichiometries were fixed to 2. Errors were calculated from at least three independent measurements to one standard deviation.