Figure 2.
The relative ATP level change upon glucose deprivation in the absence or presence of ersatzdroge. The relative level of ATP was assessed by measuring luminescence by CellTiter-Glo ATP assay system (Promega). The % value indicates the relative ATP level remaining in cells that have been glucose-deprived for 1 hr by replacing the media to glucose-free and serum-free RPMI-1640 compared to controls which were not deprived (<1 min). The ATP reduction in MCF-7/Dox was enhanced in the presence of ersatzdroge. (**, p<0.005; ***, p<0.0005)