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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: Int J Cancer. 2014 Sep 2;136(4):E188–E196. doi: 10.1002/ijc.29158

Figure 3.

Figure 3

Effect of limitation of culture media resources, glucose (A) and glutamine (B). MCF-7 and MCF-7/Dox cells were plated and cultured overnight. Cells were cultured further 48 hours after the media was changed to resource-deprived media as indicated. In order to exclude any residual resource in serum, dialyzed FBS (dFBS) was used. Cells grown in a fully supplemented medium containing regular FBS was used as a control group. The amount of survived cells in each group was measured by crystal violet staining (see Materials and Methods) and compared with the control group.