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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Exp Mol Pathol. 2014 Sep 16;97(3):359–367. doi: 10.1016/j.yexmp.2014.09.007

Figure 2. Competition of m610 with m708.5 in binding to IGF2.

Figure 2

(A) IGF2 was directly coated on the ELISA plate. Bound scFv m610 was detected by HRP-conjugated anti-Flag antibody in the presence of fixed concentration of antibody competitors (IgG1 m708.5 or IgG1 m102.4. (B) IgG1 m610.27 was coated on the ELISA plate and IGF2 was captured by coated IgG1 m610.27. Bound scFv m708.5 or VH m630.3 was detected by HRP-conjugated anti-Flag tag antibody.