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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 1;1842(12 0 0):2569–2578. doi: 10.1016/j.bbadis.2014.07.027

Figure 2.

Figure 2

The effects of db-cAMP (250 µM) on Ca2+i levels in LC neurons from neonatal rats older than P10. (A) LC neuron loaded intracellularly with the Ca2+-sensitive dye Fura-2. Rfl is the ratio of fluorescence from excitation at 340nm/ 380nm. Exposure to the membrane permeable cAMP analogue db-cAMP causes a reversible and repeatable increase in intracellular Ca2+. (B) Average values for Rfl before (left bar) and after adding db-cAMP (right bar) to the superfusate (n=3). Increases in Rfl values were significant with a P<0.005 (paired t-test).