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Immunohistochemistry studies of soluble adenylyl cyclase in LC neurons. (A) Immunohistochemical studies of LC neurons from a P10 rat incubated with an antibody for sAC and secondary antibody CY3 show the presence of the sAC enzyme in the cytoplasm (red puncta). Green fluorescence is autofluorescence from the catecholamines in the largely catecholaminergic LC neurons. (B) LC neurons from a P12 rat incubated with CY3 only. Notice the absence of red puncta. Scale bars represent 50 µm.
