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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Neuroimage. 2014 Oct 23;105:53–66. doi: 10.1016/j.neuroimage.2014.10.047

Figure 4. A Focused Comparison of Anterograde Tracing and Probabilistic Tractography in the Midbrain and Thalamus.

Figure 4

Panel A shows the BDA anterograde tracing results for injections into the MGN/S, with notable pathways to the hypothalamus (zona incerta and lateral hypothalamus) and thalamus (lateral posterior and central median nucleus). Panel B shows an extensively thresholded (50% of tracts) average tractography results, which corresponds to the Panel A, that accurately correspond to those in Panel A (indicated with white arrows). Panel C–E shows the same average probabilistic tractography, though with different threshold values. Note that Panel A has rather diffuse tracts running across the thalamus and hypothalamus, which are most closely recapitulated in Panel E with the 1% threshold. Yet even at the 1% threshold there is a loss of the auditory cortex connections that are apparent at 0.1% thresholding in Figure 4B.