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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mol Microbiol. 2014 Nov 14;94(6):1361–1374. doi: 10.1111/mmi.12839

Figure 6.

Figure 6

BSAP-1 is secreted extracellularly in outer membrane vesicles. A. Western immunoblot of 638R pMCL139 whole cell lysate, supernatant, or OMV fraction probed with polyclonal antiserum to His-BSAP-1. B. Immunofluorescence analysis of OMV fraction of 638R pMCL139 (left) or ΔBF1646 (right) using antiserum to His-BSAP-1. C. Agar spot overlay assay showing growth inhibition of strain 12905 by purified OMVs from 638R pMCL139, but not ΔBF1646. OMVs were harvested at mid-log (OD600 = 0.6) and 50 μl volumes containing 50 μg (ΔBF1646) or 52 μg (638R pMCL139) of total protein were added in 50 μl volumes to plates prior to overlay.