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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 31;94(6):1272–1284. doi: 10.1111/mmi.12828

Figure 2.

Figure 2

fliZ is required to maximize the fliC-HIGH subpopulation at late timepoints. A) A transcriptional fusion of the fliC promoter to gfp (pPfliC::gfp) was used with flow cytometry to take a census of fliC-expression in WT (BC2117) and ΔfliZ (BC2119) Salmonella populations as the cultures progressed through five hours of growth after backdilution (figure S2). B and C) Percentages of cells falling within the fliC-HIGH (B) and fliC-INT (C) gates for WT and the ΔfliZ mutant. D and E) Kinetic plots of subpopulation flux for WT (D) and ΔfliZ (E). Histograms in (A) depict the results of one representative experiment; associated subpopulation percentages in (A) are the means of three independent experiments. Line graphs in (B–D) represent the means with standard deviation of the three independent experiments. One-way ANOVA with Tukey’s multiple comparisons test was used to compare all strain/timepoint values. *p<0.05, **p<0.01, ***p<0.001.