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. 2014 Sep 1;21(6):543–554. doi: 10.1111/xen.12124

Figure 4.

Figure 4

Serum absorption of pig-to-baboon induced antibody to HEK-B4T (B4T) cells. Sensitized baboon serum was absorbed by incubating baboon serum (1 : 10 dilution) with 1 × 107 GTKO PAECs (A) or HUVECs (B) for 1 h at 4 °C. Absorption was performed 3 times for each cell type. IgG binding was analyzed by flow cytometry in dilutions from 1 : 20 to 20 : 48. (A) Closed symbols IgG binding from whole serum to GTKO PAECs (circle) and HEK-B4T cells (triangle). Open symbols are IgG binding after absorption with GTKO PAECs. Over the dilution range, absorption reduced IgG binding by an average of 89% for GTKO PAECs and 94% for HEK-B4T cells. (B) Closed symbols IgG binding from whole serum to GTKO PAECs (circle), HEK-B4T cells (triangle) and HUVEC (square). Open symbols are IgG binding after absorption with HUVECs. Absorption reduced HUVEC reactivity on average by 96%, had a minimal effect on IgG binding to GTKO PAECs (0.05%) and only a minor reduction of IgG binding to HEK-B4T (12%) cells.