Table 1.
No. | ID | Sequence | BP to exon | Site |
---|---|---|---|---|
1 | Exon1-F | AAGGCAGGACCTAGAAGGTG | 90 | flank exon 1 |
2 | Exon1-R | AACGAGCAGGAACTCTCAAC | 185 | flank exon 1 |
3 | Exon2-F | GCTGTGCAAAAGCTTGTCAGTTTGC | 186 | flank exon 2 |
4 | Exon2-R | CACAGTAAAGCCACAGGAGGAG | 41 | flank exon 2 |
5 | Exon3-F | CCCAATCTGTGATCTTTGAC | 24 | flank exon 3 |
6 | Exon3-R | GGAATGAGTAGAGAGCTTCC | 41 | flank exon 3 |
7 | Exon4-F | ATCTGGCATGGTCAGTGCATTG | 145 | flank exon 4 |
8 | Exon4-R | CAGTGGTGGAAACAGTGAG | 150 | flank exon 4 |
9 | Exon5-F | AACTCTTGGCATCCTCTC | 51 | flank exon 5 |
10 | Exon5-R | TGAGACCAGCCACCATCTCA | 73 | flank exon 5 |
11 | Exon7-F | AGTGACCCAGATACCGTG | 29 | flank exon 6 |
12 | Exon7-R | CTAAGGATCCAGTGTTGTCAC | 224 | flank exon 6 |
13 | Exon8-F | TCCACAAACACTCGAGACAG | 84 | flank exon 7 |
14 | Exon8-R | TGGGTAGTACTCACGAACACTC | n/a | in exon 8 |
15 | Exon9-F | AGAGAGTCCCTCTCCTTCTTC | 152 | flank exon 9 |
16 | Exon9-R | TCAGATGGCAATGGGGTAGAAC | 22 | flank exon 9 |
17 | Exon10-F | ACAGGCTCCTTGGATATGGAG | 36 | flank exon 10 |
18 | Exon10-R | TTGACAACACCACTGGTCACCAC | n/a | in exon 10 |
19 | Intron5-F | GTTTGAAGGACCTGATGCTC | n/a | in exon 5 |
20 | Intron5-R | TGTTCAGTGTCCCCAGAGAAG | n/a | in exon 6 |
21 | Intron6-F | ACACGAGCACAGAGTACCACC | n/a | in exon 6 |
22 | Intron6-R | AAACTTGGCCACTGAGGACTTG | n/a | in exon 7 |
23 | Intron8-F | GACAGCCACGTGGAGTATTAC | n/a | in exon 8 |
24 | Intron8-R | TGAGATATGGCCAGGTTTCTG | n/a | in exon 9 |
25 | Intron10-F | GCGACTCCAAAGAATTGGCTTC | n/a | in exon 10 |
26 | Intron10-R | TGGTGACCTATGATCACGTGTG | n/a | in exon 11 |
The table list the names, sequences, and location of primers used to confirm the genomic organization of the porcine B4GALNT2 gene. Primer numbers (No.) correspond to values in Fig.2A. Not applicable, n/a.