Table 2.
Differential immune response to HEK-B4T cells after cardiac xenotransplantation
| Donor | Survival (days) | HEK-B4T IgM | HEK-B4T IgG | GTKO PAEC IgM | GTKO PAEC IgG |
|---|---|---|---|---|---|
| GTKO:CD55 | 71 | − | + | 0.5 | 0.6 |
| GTKO:CD55 | 31 | − | − | 0.5 | 0.4 |
| GTKO:CD55 | 28 | +++ | +++ | 4.8 | 2.5 |
| GTKO:CD55 | 27 | +++ | + | 2.2 | 8.5 |
| GTKO | 22 | +++ | ++++ | 1.9 | 7.3 |
| GTKO | 21 | − | − | 1.3 | 0.9 |
| GTKO:CD55 | 18 | − | − | 0.4 | 0.4 |
Table shows the specific induced antibody response in pig-to-baboon heterotopic cardiac xenograft recipients directed to the B4GALNT2 (HEK-B4T) glycan or to GTKO PAECs. Antibody reactivity to HEK-B4T cells was estimated as described in the Materials and Methods. There was a positive and significant (P < 0.05) Spearman rank correlation coefficient between the anti-PAEC and anti-HEK-B4T IgM (r = 0.8929) and IgG (r = 0.8571) immune response.