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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Phytochem Anal. 2014 Aug 7;26(1):47–53. doi: 10.1002/pca.2535

Figure 2.

Figure 2

The SDS-PAGE gels for myrosinase-rich fractions obtained by CCC (STA) from (A) daikon, (B) mustard, (C) broccoli and (D) moringa. Fractions were concentrated about fivefold with centrifugal filters (3 K MWCO) then prepared as described in the ‘Experimental’ section. Lanes: (1) MW standards; (2) crude tissue extract in upper phase of solvent system (not utilised for chromatography); (3) crude tissue extract in lower phase of solvent system; (4–9) peak myrosinase-containing fractions; (10) myrosinase standard. Red rectangles highlight myrosinase bands.