Figure 3.

The CCC (STA) chromatograms and myrosinase activity purified from four different sources: (A) daikon – freeze-dried 7-day-old daikon sprouts (Raphanus sativus); (B) mustard – commercial ground mustard seed (Sinapis alba); (C) broccoli – freeze-dried 3-day-old broccoli sprouts (Brassica oleracea var. italica); and (D) moringa – freeze-dried leaves of the horseradish tree (Moringa oleifera). Dry plant material was comminuted into equal portions of the solvent system upper and lower phases, which were vigorously mixed, then centrifuged, and only the lower phase was used for CCC with inline de-salting of the eluent prior to detection by absorbance at 280nm as described in the ‘Experimental’ section (solid line; relative scaling). Myrosinase activity (dashed red line) and specific activity (dotted blue line) are plotted against a common axis.