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Determination of partition coefficients for daikon myrosinase
| Solvent systema | 32% PEG-1000 | 100% PEG-400 | 25% potassium phosphate (12.5% each, K2HPO4 and KH2PO4) |
Myrosinase partition coefficient (Kup/lp) |
|---|---|---|---|---|
| 1b | 1 | 0 | 1 | 0.08 |
| 2 | 4 | 1 | 15 | 0.03 |
| 3 | 3 | 2 | 10 | 0.33 |
| 4 | 2 | 3 | 10 | 1.27 |
| 5 | 1 | 4 | 15 | 5.27 |
| 6 | 0 | 5 | 30 | 6.57 |
Kup/lp, partition coefficient of upper phase to lower phase.
Stock solutions prepared by weight (w/w).
System that we ultimately chose for myrosinase purification.
