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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Neurol. 2014 Oct 18;263:141–149. doi: 10.1016/j.expneurol.2014.10.004

Figure 2.

Figure 2

A, p-GSK-3β expression was significantly higher 48hrs after HI which was prevented by the GSK-3β siRNA. B, GSK-3β expression was unaffected 48hrs after HI but the GSK-3β siRNA significantly reduced its expression. C, Bcl-2/Bax ratio was significantly reduced 48hrs after HI which returned to the Sham level with the GSK-3β siRNA. D, CC3 expression 48hrs after HI was significantly elevated and was prevented by the GSK-3β siRNA. D, Representative Western blots of p-GSK-3β, GSK-3β, Bcl-2, Bax and CC3. n=6/group. E, Representative microphotographs of immunofluorescence staining showing colocalization of CC3 (green) and p-GSK-3β (red) 48hrs after HI. n=3/group. Arrows show representative cells with p-GSK-3β and CC3 co-localization. Scale bars=25μm. * p<0.05 vs. Sham, # p<0.05 vs. HI, † p<0.05 vs. HI + GSK-3β siRNA.