Fig. 4.
Relative mRNA levels of selected genes in livers of Pnpla3148M/M and Pnpla347A/A mice with wild-type littermates (n = 4/group). The mice used in this experiment are described in the legends to Figs. 2 and 3. mRNA levels were quantified by real-time PCR, normalized to levels of 36B4, and expressed relative to levels in wild-type mice. Values are means ± SEM. SREBP1c, sterol regulatory element binding protein 1 isoform C; SREBP2, sterol regulatory element binding protein 2; LXRα, liver X receptor alpha; PGC-1α, PPARγ coactivator 1α; PPARα, peroxisome proliferator-activated receptor alpha; PPARγ, peroxisome proliferator-activated receptor gamma; ChREBP, carbohydrate-responsive element-binding protein; L-PK, liver pyruvate kinase; PEPCK, phosphoenoylpyruvate carboxykinase; ACC1, acetyl-CoA carboxylase-1; ACC2, acetyl-CoA carboxylase 2; FAS, fatty acid synthase; SCD1, stearoyl-CoA desaturase-1; ACL, ATP citrate lyase; ELOVL6, ELOVL family member 6; TNFα, tumor necrosis factor alpha; COL1α1, collagen type-1α1; ACTA2, α-smooth muscle actin; AOX, acyl-CoA oxidase-1; LCAD, long-chain acyl-CoA dehydrogenase; MCAD, medium-chain acyl-CoA dehydrogenase; CPT1, carnitine palmitoyltransferase1; ATGL, adipose triglyceride lipase; MTTP, microsomal TG transfer protein; PLIN2, perilipin 2; G0S2, G0/G1switch 2; CGI-58, comparative gene identification 58; AGPAT1-3, 1-acylglycerol-3-phosphate O-acyltransferase 1-3; GPAT, glycerol-3-phosphate acyltransferase; DGAT1, diglyceride acyltransferase-1; DGAT2, diglyceride acyltransferase-2; MGAT1, monoacylglycerol O-acyltransferase 1; CFD, complement factor D (adipsin); CIDEC, cell death-inducing DFFA-like effector C. The experiment was repeated twice and the results were similar.