Fig. 7.
PNPLA3 on lipid droplets from fat-fed mice. Livers were harvested from 17-week-old female wild-type (+/+) and Pnpla3148M/M mice (n = 5/group) fed a high-fat diet for 12 weeks. (A) Hepatic PNPLA3 mRNA levels were determined using real-time PCR, as described in the Materials and Methods. (B) Hepatic TG levels were measured by enzymatic assay as described in the Materials and Methods. (C) Immunoblot analysis of lipid droplets isolated from 4 mice per group was performed as described in the legend to Fig. 6, and (D) quantified as described in the Materials and Methods. Values are means ± SEM. *P < 0.05.