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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Biotechnol. 2014 Sep 3;32(12):1262–1267. doi: 10.1038/nbt.3026

Figure 1.

Figure 1

sgRNA activity screens in mouse and human cells. (a) Representation of the sgRNA libraries. Colors represent genes assayed by FACS; light gray indicates genes either poorly expressed or not assayed; dark gray indicates targets not found in the mouse or human genomes. (b) Top: Antibody staining in cells (red) compared to unstained cells (black). Bottom: FACS plots indicating the negative population isolated for each cell surface marker after library transduction. (c) Percent of sgRNAs enriched >10-fold (mouse) or >2-fold (human) in the marker-negative population that were on-target.