Table 2.
Gene-specific primers used for validation
| Gene name | GenBank accession number | Primer sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| GCET2 | NM_152785 | F: ACCCTCATCAATCATCGGGTT | 122 |
| R: TCAGTCTCAGTTCCTCCCAAG | |||
| CFTR | NM_000492 | F: TGCCCTTCGGCGATGTTTTT | 127 |
| R: GTTATCCGGGTCATAGGAAGCTA | |||
| FOXP1 | NM_032682 | F: TCCCGTGTCAGTGGCTATGAT | 226 |
| R: CTCTTTAGGCTGTTTTCCAGCAT | |||
| GARS | NM_002047 | F: TTGGCCCAGCTTGATAACTATG | 103 |
| R: ACACTGGAGGGGATAGATCATTT | |||
| GAPDH | NM_001256799 | F: ACAACTTTGGTATCGTGGAAGG | 101 |
| R: GCCATCACGCCACAGTTTC |
F forward primer, R reverse primer