Figure 1.

Bim is critical for the initial contraction of KLRG1^hiCD127^lo effector CD8⁺ T cells. Groups of WT and Bim^−/− mice (n=4–6 mice per genotype per time point) were infected intraperitoneally (i.p.) with 2 × 10⁵ p.f.u./mouse LCMV and killed 10, 15, 24 or 40 days post LCMV infection. Splenocytes were stained with D^b-GP33 tetramers and antibodies against KLRG1, CD127, CD8 and CD44 and analyzed by flow cytometry. D^b-GP33-specific CD8⁺ CD44⁺ cells were gated. (a) The graphs show the total numbers±S.E.M. of KLRG1^hiCD127^lo, KLRG1^hiCD127^hi or KLRG1^loCD127^hi subsets on indicated days after infection. (b) Representative dot plots for KLRG1 and CD127 are shown after gating on CD8⁺ GP33⁺ cells in each group on indicated days. (c) Graphs show percentages of CD8⁺ GP33⁺ KLRG1^hiCD127^lo or KLRG1^loCD127^hi subsets on indicated days after infection. Data are representative of two independent experiments. P values for statistically significant differences were calculated by Student's t-test and *P≤0.05 and **P≤0.01