Figure 6.

L-GILZ inhibits p53-dependent proliferation and tumor growth. p53^+/+ or p53^−/− HCT116 cells (0.25 × 10⁶) were transfected with L-GILZ or empty vector, and 24 h after transfection, these cells were (a) seeded in triplicate in 96-well plates for [³H]-thymidine assays (*L-GILZ-transfected versus empty vector) or (b) plated in 10-cm dishes in triplicate for clonogenic assays (***L-GILZ-transfected versus empty vector) as described in Materials and Methods. Number of colonies represents the mean±S.D. of three independent experiments. Representative plates for each group are shown. (c) p53^+/+ or p53^−/− HCT116 cells (5 × 10⁶) transfected with L-GILZ or empty vector were subcutaneously injected into the right flank of nude mice. Tumor volume (n=5 per group) was determined in vivo at the indicated time points using an external caliper. Mice were killed 40 days after injection, and tumors were excised, imaged and weighed. *L-GILZ- versus empty vector-transfected cells