Figure 5.

Loss of TRIB3 enhances the incidence of premalignant and malignant lesions in Pten^+/− mice. (a) Effect of the genetic inactivation of TRIB3 on the incidence of premalignant and malignant lesions in the adrenal glands of Pten^+/− mice. Data are expressed as the percentage of animals of each genotype that exhibit hyperplasia of the adrenal glands (upper panel) or pheocromocytomas (middle panels). Bottom panel: Representative microphotographs ( × 10) of the different lesions observed in the adrenal glands of Pten^+/− Trib3^+/− and Pten^+/− Trib3^−/− animals stained with hematoxilin/eosin. (b) Representative microphotographs of anti-phospho AKT and total Akt immunostaining of the adrenal glands of WT, Pten^+/− and Pten^+/− Trib3^−/− animals. Note that the hyperplasic region located in the medulla of the adrenal gland exhibits strong phospho-AKT immunostaining in Pten^+/− Trib3^−/− mice but not in Pten^+/− mice (n=3; × 40). Insets show details of the different panels at higher magnification. See also Supplementary Figure S5