The splicing efficiency of dmpi8 regulates daytime but not nighttime sleep in daily light-dark cycles. (A-L) Shown are group averages for the percentage of flies sleeping during 4-h time-windows throughout a 24-h day for either p{dmper/8:8} [indicated as wild-type (WT)] (black line) or p{dmper/ M2M1} (dashed black line) adult flies maintained at the indicated temperatures (right of panels) and light/dark conditions (top of panels). Results shown are for either male (A-F) or female (G-L) flies. For each genotype, gender, and entrainment condition, data from at least 16 flies was used to generate the sleep profiles shown. LD, 12-h light/12-h dark; DD, constant darkness. For LD, sleep data from the last 3 days were pooled, whereas for DD, only data from the first day were used. White, black, and dark gray horizontal bars denote periods of light, dark, and “subjective daytime” in DD, respectively. Values for p{dmper/8:8} (WT) and p{dmper/M2M1} flies are significantly different using student's t-test; * P < 0.05; ** P < 0.005.