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. 2014 Dec 3;15(12):525. doi: 10.1186/s13059-014-0525-9

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© Saadatpour et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Experimental validation of the functional difference between the two leukemic cell subtypes. (A) Snapshot of a colony-forming assay of CD24- leukemic cells on day 7. (B) Snapshot of a colony-forming assay of CD24+ leukemic cells on day 7. (C) Number of spread-type colonies of different leukemic cell subpopulations. (D) Number of sphere-type colonies of different leukemic cell subpopulations. (E) Total number of cells after one-week culture in methylcellulose supplemented with IL3, IL6, IL7 and stem cell factor. The error bars represent standard deviation.