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. 2014 Nov 18;15(11):519. doi: 10.1186/s13059-014-0519-7

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© Matranga et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Depletion of rRNA from human LASV isolates. (A) Rarefaction analysis of LASV sample (ISTH2016) from a rRNA-depleted (gray) or control (undepleted, blue) preparation. Data best fit (dashed line) to the Michelis-Menten formula in which projected saturation value equals V_max (see Materials and methods). (B) LASV genomic coverage from a LASV sample (ISTH0073) from a rRNA-depleted (gray) or control (blue) preparation. L, S segment, Z, L, NP, GPC: boundaries of each LASV genomic segment with specified genes encoded on each segment. (C) Starting overall content (RNA input) and enrichment of unique LASV (Library content) upon rRNA depletion from nine different clinical isolates.