Figure 7. Mouse Il33r and Dusp1 are suppressed by H. polygyrus exosomes and the secreted miRNA repress target sites in Dusp1.

(a) Volcano plot of mouse genes up- or downregulated upon incubation with H. polygyrus-derived exosomes; red=FDR P<0.05 and FC>30%. (b) Levels of Dusp1 and Il1rl1 in mouse epithelial cells (5 × 10⁴) following 48 h treatment with 5 μg H. polygyrus exosomes or MODE-K-derived exosomes, n=8, error bars are mean±s.e.m. Data analysed by ANOVA and Tukey’s post test, *P<0.05, **P<0.01, ***P<0.005, ****P<0.001. (c) Repression of Psicheck reporter vector containing Dusp1 or Il1rl1 3′UTRs fused to Renilla luciferase by co-transfection with individual or pooled synthetic H. polygyrus miRNAs (50 nM), data represent renilla/luciferase ratios, normalized to the values obtained for untreated samples; n=3, ***P<0.005, *P<0.05.