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. 2014 Nov 21;5:5519. doi: 10.1038/ncomms6519

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GhRDL1 and GhEXPA1 promoters were fused to the LUC reporter, respectively, and the promoter activities were determined by a transient dual-LUC assay in Nicotiana benthamiana. The relative LUC activities were normalized to the reference Renilla (REN) luciferase. The corresponding effector (+), empty vector (−) or neither (NA) were co-filtrated (data are presented as mean±s.e.m., n=3, *P<0.05; **P<0.01, Student’s t-test). (a) Effects of GhHOX3 and GhHD1 on activities of RDL1 and EXPA1 promoters. (b) Effects of L1-box mutation on the activity of RDL1 and EXPA1 promoters. The L1-box was mutated (mutant) as shown (Fig. 2e). (c) Inhibitive effects of GhSLR1 on activation of RDL1 and EXPA1 promoters by GhHOX3 and GhHOX3+GhHD1.