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Fumonisin production and transcript of the key biosynthetic gene FUM1 in vitro. (A) Quantification of the main B-series fumonisins (FBtot = FB1 + FB2 + FB3, expressed as ppb) and (B) FUM1 mRNA quantification in the WT, mutant (ΔFvlds1D) and COM strains of F. verticillioides relative to inoculation time 0. Fungi were grown for 2–15 DAI in FB-inducing CDYM; results are the mean (± SE) of six replications deriving from two independent experiments.
