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. 2014 Dec 11;5:669. doi: 10.3389/fmicb.2014.00669

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Copyright © 2014 Scala, Giorni, Cirlini, Ludovici, Visentin, Cardinale, Fabbri, Fanelli, Reverberi, Battilani, Galaverna and Dall'Asta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–F) mRNA levels of fungal and plant genes in infected cobs. F. verticillioides LDS1 (A), LDS2 (B), LDS3 (C), and LOX (D), Zea mays LOX3 (E), and PR4 (F) were quantified at 2–15 DAI in maize cobs non-inoculated (mock), or inoculated with the WT or mutant strain (ΔFvlds1D). Relative mRNA expression is calculated by using the 2^−ΔΔCt method, i.e., by normalizing gene of interest expression onto housekeeping gene expression and to their value at the time of inoculation (time zero). Results are the mean (± SE) of six replications deriving from two independent experiments.