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. 2014 Dec 11;5:695. doi: 10.3389/fmicb.2014.00695

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Copyright © 2014 Näther-Schindler, Schopf, Bellack, Rachel and Wirth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Repolymerization of denatured flagella. (A) SDS-PAGE: Flagella purified by CsCl-gradient centrifugation were denatured into monomeric flagellins by SDS and heat denaturation (lane 1). After extensive dialysis against 5 mM HEPES buffer only single flagellins were observed (lane 2). The denatured flagellins were used for polymerization assays at: 8°C (lanes 3 and 7), 37°C (lanes 4 and 8), 60°C (lanes 5 and 9), and 90°C (lanes 6 and 10). Analysis was done after 1 (lane 3–6) or 6 days (lane 7–10) of incubation. (B–D) show TEM analyses of: (B) the flagella preparation; (C) denatured flagellins (lane 2); (D) the result from a 90°C repolymerization after 1 day (lane 6). Size bars are 100 nm, each.