. 2014 Dec 20;192:393–399. doi: 10.1016/j.jbiotec.2014.04.008

Table 2.

Relative Gibbs free energies refer to back and forward reactions compared to enzyme activities.

Reaction	$Δ G_{rxn}^{0}$ ^a (kcal/mol)	Enzyme activity (U/mg)
2-Cyclohexen-1-ol + NADP⁺ → 2-Cyclohexen-1-one + NADPH	−0.0143	0.91
2-Cyclohexen-1-one + NADPH → 2-Cyclohexen-1-ol + NADP⁺	+0.0143	0.29
Cyclohexanol + NADP⁺ → Cyclohexanone + NADPH	+1.507	1.59
Cyclohexanone + NADPH → Cyclohexanol + NADP⁺	−1.507	30.08

$Δ_{rxn} G^{0} = \sum Δ_{f} G_{products of rxn}^{0} - \sum Δ_{f} G_{reactants of rxn}^{0};$ Δ_fG⁰ were estimated based on GCM software (http://lcsbweb.epfl.ch/GCMWebSite/Login.php) (Jankowski et al., 2008, Mavrovouniotis, 1990, Mavrovouniotis, 1991).