Figure 3. Nanoceria inhibit the activation of MAP kinases in the retina of Vldlr−/− mouse.

Wt (n=3) and Vldlr−/− (n=3) mice were injected with 1μl of 1 mM nanoceria on P28 and the retinas were collected at P35. Uninjected Wt (n=3) and Vldlr−/− (n=3) mice at P35 served as controls. Retinal proteins (10μg) were subjected to 10% SDS-PAGE and Western blot analysis for the detection of phosphorylated p-ERK 1/2 (A), p-JNK 1/2 (B) or p-p38 (C) MAP kinases. The same membranes were then stripped and reprobed with anti-ERK 1/2, JNK 1/2 or p38 antibodies, which detect total protein levels, or anti-actin antibody, respectively. The blots were scanned, densitometry analysis performed, and the normalized results are presented as the mean ± S.E.M of the p-ERK/Actin (D), p-JNK/Actin (E), p-p38/Actin (F), ERK/Actin (G), JNK/Actin (H) and p38/Actin (I) ratio from 3 independent experiments.