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. Author manuscript; available in PMC: 2014 Dec 11.
Published in final edited form as: Exp Eye Res. 2013 Aug 24;116:63–74. doi: 10.1016/j.exer.2013.08.003

Figure 4. Nanoceria inhibit the activation of Akt in the retina of Vldlr−/− mouse.

Figure 4

Wt and Vldlr−/− mice were injected with 1μl of 1 mM nanoceria on P28 and the retinas were collected at P35. Uninjected Wt and Vldlr−/− mice at P35 served as controls. Retinal proteins (10μg) were subjected to 10% SDS-PAGE and Western blot analysis for the detection of phosphorylated p-Akt (A). The same membranes were then stripped and reprobed with anti-Akt antibody which detects total protein levels, or anti-actin antibody, respectively. The blots were scanned, densitometry analysis performed, and the normalized results are presented as the mean ± S.E.M of the p-Akt/Actin (B) and Akt/Actin (C) ratio from 3 independent experiments.

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