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. 2014 Sep 28;21(6):673–683. doi: 10.1093/dnares/dsu029

Figure 1.

Figure 1.

Karyotyping of the Vero JCRB0111 cell line. (A) Chromosome number in the Vero cell line based on 100 Giemsa-stained metaphases, which showed that the modal number was 59 chromosomes and differences in the chromosome number between 52 and 62 indicated heterogeneous karyotypes. (B) G-banded karyotype of Vero cells with 59 chromosomes consisted of 16 homologous pairs (blue numbers) and 13 abnormal chromosomes (black numbers). (C and D) M-FISH signal pattern using human probes on a normal AGM metaphase (C), and a main clone of the Vero cell line (D). Paints of human chromosomes 1–22 and X showed homologous regions in the AGF and the identity of 32 and 40 syntenic blocks in a normal female and Vero, respectively. The number on the right of aberrant chromosomes (D) shows correspondence to AGF chromosomes. The derivative chromosome der(13) (white arrow in D) showed an extra signal that corresponded to AGM chromosomes 15 and 22, both of which were painted by human chromosome 3. Although the addition in der(13) could not be distinguished using human M-FISH probes, the copy number analysis revealed a gain at chromosome 15q (Fig. 2B), which indicated that der(13) had additional material over chromosome 15.