Table 2.
Summary statistics from Illumina MiSeq sequencing of GH11 PCR fragments amplified, for each four cDNA samples, before (H0) or after one (H1) or two (H2) hybridization capture
| Sample | Total no. of sequences | Total no. of clustersa (95%) | No. of clusters encompassing ≥90% of the sequences | Shannon diversity index (H′)b | No. of shared clusters between H0–H1–H2b |
|---|---|---|---|---|---|
| PUE_H0 | 12,960 | 298 | 52 (17%) | 3.819 | 70 (11%) |
| PUE_H1 | 24,565 | 227 | 51 (22%) | 4.015 | |
| PUE_H2 | 25,053 | 291 | 46 (16%) | 3.912 | |
| BRE_H0 | 13,538 | 87 | 9 (10%) | 2.254 | 11 (5%) |
| BRE_H1 | 42,000 | 140 | 5 (4%) | 1.651 | |
| BRE_H2 | 46,626 | 112 | 6 (5%) | 1.73 | |
| BRH_H0 | 2,765 | 26 | 3 (12%) | 1.061 | 5 (4%) |
| BRH_H1 | 28,366 | 51 | 3 (6%) | 1.234 | |
| BRH_H2 | 17,322 | 159 | 18 (11%) | 2.135 | |
| BEW_H0 | 41,799 | 214 | 15 (7%) | 2.761 | 38 (6%) |
| BEW_H1 | 42,308 | 249 | 10 (4%) | 2.496 | |
| BEW_H2 | 36,859 | 205 | 6 (3%) | 2.196 |
aIncluding singletons.
bShannon diversity indices and shared clusters were calculated after rarefying the different data sets from the same soil to the same sequencing depth of 12,960, 13,538, 2,765 and 36,859 sequences for PUE, BRE, BRH and BEW, respectively.