Figure 2. Complementation assay by in vivo EPs.

A, Equator images of Sun1 ^−/− spermatocytes expressing ^GFP-SUN1 or SUN1^-MYC stained for telomere TRF1 (green), GFP or MYC (blue) and SCP3 (red). The graph shows the number of internal telomeres (TRF1 foci) in wild type (zygotene to pachytene), Sun1 ^−/− and Sun1 ^−/− expressing exogenous SUN1 protein. B, Quantification of TRF1 foci number in wild type, Sun1 ^−/− spermatocytes and Sun1 ^−/− spermatocytes expressing exogenous SUN1 protein. C, Representative pictures of Sun1 ^−/− spermatocytes or Sun1 ^−/− spermatocytes expressing SUN1^-MYC stained for SCP3 (red), SCP1 (green) and MYC (blue). The median numbers are shown in the graphs. Bars, 5 µm.