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. 2014 Dec 11;10(12):e1004851. doi: 10.1371/journal.pgen.1004851

Figure 3. Knockdown of Cbx8 reduces transcription of activated target genes.

Figure 3

(A) The mRNA levels of Cbx8 in control cells (sh Scr) and after shRNA-mediated knockdown of Cbx8 using lentiviral transduction of two independent hairpins (#1 and #2) were analyzed by qRT-PCR before and after treatment with RA. Error bars denote s.d., and n = 3. (B) Western blot analysis of Cbx8 in RA-treated ES cells. Histone H3 was used as loading control. (C) Transcript expression levels of genes in cells expressing Cbx8-specific shRNAs or control shRNA (indicated by minus) were analyzed. Color code of target genes is according to Figs. 1F and 2B. For each gene, values are plotted relatively to control treated with RA. Error bars denote s.d.; n = 3; *, p<0.05 (comparing to RA-treated control). (D) The occupancy of Cbx8 on the same genes was analyzed by ChIP. Error bars denote s.d., and n = 3.