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. 2014 Dec 11;10(12):e1004851. doi: 10.1371/journal.pgen.1004851

Figure 7. Cbx8 binds to persisting K27 methylation during differentiation.

Figure 7

(A) Trimethylation of lysine 27 of Histone H3 was analyzed by ChIP during a time course of RA-induced differentiation of ES cells. Control ChIPs with IgG are shown in the top panel on the same scale. Error bars denote s.d.; n≥3; n.d.  =  not done. (B) ChIP of H3K27me3 (+) and IgG (-). For each Cbx8 target gene values are plotted relatively to control cells. Enrichments on-target genes are plotted relative to the average enrichment on all six target genes in control cells. Error bars denote s.d.; n = 3. (C) ReChIPs using anti-K27me3 antibody and IgG as control were performed on material enriched in a primary ChIP with anti-Cbx8 antibody. Data is plotted relative to IgG. Error bars denote the variation of the mean of two independent experiments.