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. 2014 Dec 11;10(12):e1004859. doi: 10.1371/journal.pgen.1004859

Figure 2. Hyper-recombination and genetic interactions of rem mutations with recombination and replication functions.

Figure 2

(A) Analysis of Rad52 foci after exposure to UV-C. Only cells in S and G2 were considered. Error bars represent the SD of three independent experiments. One representative experiment is shown for rad3-102 in order to facilitate comparison with previous published data [15]. (B) Analysis of genetic interactions of rem alleles of RAD3 with mre11Δ, rad51Δ and pol32Δ. Tetrads dissected on rich medium are shown. The sites of double and triple mutants are indicated by triangles and squares, respectively. (C) Survival curves of WT, rad3-101, rad51Δ and their corresponding double mutant cells after UV-C exposure. (D) FACS profiles of mid-log cultures from WT, rad3-101, rad51Δ and their corresponding double mutant cells taken every 2 h after addition of 40 mM HU.